Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Ferroptosis and intrinsic drug induced liver injury by acetaminophen and other drugs: a critical evaluation and historical perspective EurekAlert! About Acetaminophen Overdose Acetylcysteine for Acetaminophen Poisoning New England Journal of Medicine Sex differences in glutathione metabolism and acetaminophen toxicity